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Figure 1 | Journal of Neuroinflammation

Figure 1

From: Activation of microglial cells triggers a release of brain-derived neurotrophic factor (BDNF) inducing their proliferation in an adenosine A2A receptor-dependent manner: A2A receptor blockade prevents BDNF release and proliferation of microglia

Figure 1

Effect of LPS on the intracellular levels of pro- and mature BDNF in murine N9 microglial cells. (A) N9 cells were exposed to LPS (100 ng/mL) for 3, 6 and 12 hours, then lysed and homogenized for Western blot analysis of pro- (open bars) and mature BDNF (filled bars) immunoreactivities (37.5 and 13 kDa, respectively). Results are expressed as mean ± SEM of n (as indicated in each bar) independent experiments (*** P < 0.001, compared with non-treated cells, using the Newman–Keuls multiple comparison test) and 100% represents the pro- and mBDNF in cells that were not exposed to LPS. (B) Representative blot of the LPS (100 ng/mL for 6 hours)-mediated decrease of intracellular mature BDNF and its inability to interfere with the intracellular levels of pro-BDNF; the blots compare BDNF immunoreactivity from cells exposed (+) or not (−) to LPS. BDNF, brain-derived neurotrophic factor; LPS, lipopolysaccharide; mBDNF, mature protein BDNF; SEM, standard error of the mean.

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Journal of Neuroinflammation

ISSN: 1742-2094