Impaired mucosal immune response in MyD88
mice infected with
T. gondii. (A) Macroscopic observation of MyD88+/+ and MyD88−/− MLNs of mice infected with 80 cysts of the 76 K T. gondii strain 13 days postinfection. (B) qRT-PCR evaluation of the expression of chemokine mRNA in MLNs of MyD88+/+ (open bars) and MyD88−/− (solid bars) BALB/c mice 13 days postinfection. Results are presented as the fold increase expression of mRNA in infected mice versus expression of mRNA in non-infected mice. The results are representative of two experiments with 7 mice per group. (C) cytokine levels in supernatants of MLN cells restimulated by Toxoplasma extract from MyD88+/+ and MyD88−/− BALB/c mice 13 days postinfection. Supernatants were collected between 48 and 96 hours to determine cytokines levels (IL-10 at 72 h, IL-2 at 48 h, IFN-γ at 96 h and IL-12 p40 at 48 h) in MyD88+/+ (open bars) and MyD88−/− (solid bars) mice. These results are representative of two independent experiments with 7 mice per group. Data analyzed using a Mann–Whitney test * (P <0.05). MLN, mesenteric lymph node; qRT-PCR, real-time reverse-transcription polymerase chain reaction.