Effect of hypothermia on signal transducer and activator of transcription 3
(STAT3) phosphorylation and effect of a STAT3 inhibitor. (A) Western blot analysis of phosphorylated STAT3 (Ser727) was performed. After 4 hours of oxygen glucose deprivation (OGD), phosphorylation reached a peak with hypothermia. Relative levels of phosphorylated STAT3 are expressed as the mean ± S.E.M. of three experiments. *P <0.05 compared to the control; #P <0.05 compared to normothermia. (B) bEnd.3 cells were treated with either vehicle or JSI-124 (0.1 and 1 μM) 30 minutes before OGD. The expression of metallothionein (MT) mRNA was analyzed by reverse transcription polymerase chain reaction (RT-PCR). JSI-124 inhibited the induction of MT expression. Values represent the mean ± S.E.M. of three different experiments. *P <0.05 compared to the control; #P <0.05 compared to normothermia.