Effect of RA on TNF-
induced increase of p50/p65 binding to DNA. SH-SY5Y cells were pre-incubated with RA (9 μM) for 22 h, then incubated with TNF-α (20 ng/ml) for 2 h. The DNA binding activity of NF-κB (p50/p65) was determined using an ELISA kit. RA indicates rosmarinic acid. Results are expressed as fold increase over control, n = 5. *
P <0.01 vs. TNF-α-induced group. #
P <0.01 vs. control group. Significance was determined by one-way analysis of ANOVA followed by Dunnett’s test.