Figure 1
From: Macrophages in inflammatory multiple sclerosis lesions have an intermediate activation status
Characterization of monocyte-derived macrophages. Monocytes were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors and were cultured for 7Â days in the presence of 5% normal human serum. A gate was positioned around living (7-aminoactinomycin (7-AAD) negative) cells (approximately 70%). Of the living cells approximately 97% stained positive for CD68. Representative fluorescence-activated cell sorting (FACS) plots are shown.