Figure 3

Assessment of human astrocyte IGF1 and IGF2 mRNA expression by Q-PCR. Astrocyte cultures were treated with IL-1β (±IFNγ) IL-13, IL-4, poly(I:C) or medium alone for 6 h and Q-PCR was performed for IGF1, IGF2, and TNFα. Fold changes over control were calculated in each case and values from two astrocyte cases are shown (bar = mean value). No significant changes were noted in astrocyte IGF1 mRNA following treatment with inflammatory stimuli (A) but IGF2 mRNA was potently induced by IL-1β (±IFNγ) (B). TNFα mRNA was induced maximally by IL-1β/IFNγ and less by IL-1β or poly(I:C) but not by IL-13 or IL-4 (C).