Figure 1

Skeleton analysis of microglia morphology. A Confocal images were acquired in brain regions 1–4 (1 striatum; 2–4 cortex) as illustrated in the TTC image of a typical area of injury after ischemic stroke and 24 h reperfusion (white tissue is necrotic, and red tissue is healthy). Brain regions were conserved across time points in the ipsilateral, contralateral and sham hemispheres. B Maximum intensity projections of confocal images were converted to binary images and then skeletonized. The number of microglia process endpoints (blue) and process length (orange) were summarized for statistical comparisons from Analyze Skeleton plugin by Image J. C Composite images of neurons, microglia and cell death from ipsilateral brain regions 1–4 [blue-neuron (anti-NeuN), green-microglia (anti-iba-1), red-cell death (propidium iodide)]. Scale bar = 10 μm.