Figure 4

Microglia ramification is significantly decreased in all ipsilateral regions after ischemic stroke and 24-h reperfusion. A Representative confocal maximum intensity projection images of iba-1 positive microglia and magnified skeletonized inset in brain regions (1 striatum; 2–4 cortex) after 60 min of focal ischemia and 24-h reperfusion (Figure 1A). B Microglia process endpoints/cell are significantly reduced in ipsilateral regions 1–4 versus matching contralateral and sham regions and also significantly reduced in contralateral region 1 versus matching sham. C Microglia process length/cell is significantly decreased in all ipsilateral regions (1–4) versus matching contralateral regions. Ipsilateral microglia process length/cell was significantly reduced versus sham in regions 1, 2 and 4, and no significant differences were detected in contralateral regions versus matching sham. n = 6/sham group; n = 10/contralateral and ipsilateral groups. Data were tested for statistical differences between ipsilateral, contralateral and sham regions using one-way ANOVA and Bonferroni post-hoc testing (*p < 0.05, **p < 0.001, ***p < 0.0001). All data are mean ± SEM. Scale bar = 10 μm.