Figure 6

Microglia ramification and activity are decreased in the peri-infarct region after ischemic stroke and 24-h reperfusion. A Exemplary maximum projection intensity images of live microglia in brain regions 2 (ipsilateral peri-infarct region), 4 (ipsilateral distal to necrotic core) and C (contralateral region). Brain regions for image acquisition in relation to cerebral injury are as shown in a representative TTC image. B Sholl analysis plot of microglia. C Microglia process movement is significantly decreased in region 2 (ipsilateral peri-infarct) after ischemic stroke and 24-h reperfusion. D Sholl analysis data summary. Microglia nearest to the infarct core have a significantly increased soma size and decreased maximum branch length, number of branch endpoints, process maximum and ramification index when compared to cells in the contralateral hemisphere. Sample size is as listed in the figure. Data were tested for statistical differences between groups, using one-way ANOVA and Bonferroni post-hoc testing (*p < 0.05, **p < 0.001, ***p < 0.0001). All data are mean ± SEM. Scale bar = 5 μm.