Figure 3

Nuclear translocation of transcription factor NF-κB is not associated with ghrelin-mediated inhibition of IL-6 release. NF-κB has been implicated in ghrelin-mediated inhibition of cytokine release in murine macrophages. To determine if a similar mechanism exists in SN4741 neurones Western blots were performed on cytoplasmic and nuclear cell extracts following treatments. Under basal conditions p65 was abundant in the cytoplasmic extract (a), whereas ghrelin treatment seemingly promoted shuttling of p65 into the nuclear compartment (b). However, while LPS treatment alone elevated p65 (c) and ghrelin/[D-Lys3]-GHRP-6 treatments attenuated p65 (d, e), there was no apparent alteration in cytoplasmic to nuclear ratio to indicate regulation via p65. The blots are representative of two independent experiments.