Figure 5
Blood-brain barrier leakage evaluated by mouse IgG staining. (A) Representative images of blood–brain barrier leakage of sham- and brain-injured animals, identified by IgG staining in the brain regions underlying the craniotomy and impact site; (B) Quantitation of the area (mm2) stained with IgG (above the pre-set intensity level) at 1, 3 and 7 days after sham injury or central fluid percussion injury (cFPI), presented as means ± SD. The IgG staining was more intense in sham-injured controls at 3 days compared the 1 and 7 days post-injury time points, although without reaching statistical significance. *P<0.05; **P<0.01; ***P<0.001 compared to sham-injured controls. PID: Post-injury day.