Table 1 Overview of the primary and secondary antibodies
Antibody | Host animal/concentration | Target | Supplier |
|---|---|---|---|
β-APP | Rabbit, 1:200 | Amyloid precursor protein | Invitrogen Life Technologies Europe, BV, Netherlands |
Vimentin | Goat, 1:200 | Astrocytes | Santa Cruz Biotechnology, Inc. CA, USA |
GFAP | Rabbit, 1:500 | Astrocytes | Dakocytomation A/S Glostrup, Denmark |
MAC-2 | Rat, 1:200 | Microglia | Cederlane, Ontario, Canada |
GR-1 | Rat, 1:200 | Neutrophils | Biolegend, San Diego, USA |
CD3 | Hamster, 1:100 | T-Cells | AbSerotec, Dusseldorf, Germany |
Biotinylated anti-mouse IgG | Mouse, 1:100 | Blood brain barrier leakage | Vector laboratories, Burlingame, CA, USA |
Biotinylated anti-rabbit IgG | Rabbit, 1:200 | Secondary antibody | Vector laboratories |
Biotinylated anti-goat IgG | Goat, 1:200 | Secondary antibody | Vector laboratories |
Biotinylated anti-rat IgG | Rat, 1:200 | Secondary antibody | Vector laboratories |
Biotinylated anti-hamster IgG | Hamster, 1:200 | Secondary antibody | Vector laboratories |