Figure 6

DJ-1−/− display blunted neuroinflammatory responses in midbrain compared to wild-type mice after repeated low-dose systemic lipopolysaccharide administration. Quantitative PCR analyses of microdissected brain tissue was used to measure expression levels of the neuroinflammation markers TNF and CD45 in (A) the ventral midbrain and (B) the cortex of mice treated with low-dose systemic lipopolysaccharide (LPS) (or saline) for 6 months (mo). Parkin mRNA was also measured and found to be similar in both genotypes (data not shown). *,**Significant difference from wild-type saline; #Significant difference from DJ-1−/− saline; +Significant difference between LPS conditions. Bars represent mean ± SEM; n = 3 to 4 per group. A two-way analysis of variance was performed with Tukey’s HSD post hoc at P < 0.05 (midbrain TNF treatment effect: F_(1,9) = 16.57, P = 0.0028, genotype effect P = 0.29; midbrain CD45 treatment effect: F_(1,10) = 9.38, P = 0.0120, genotype effect P = 0.09; cortex TNF treatment effect: F_(1,10) = 6.73, P = 0.0268, genotype effect P = 0.73; cortex CD45: not significant (n.s.), treatment P = 0.679, genotype P = 0.97).