Hippocampal gene expression dysregulation of Klotho, nuclear factor kappa B and tumor necrosis factor in temporal lobe epilepsy patients

Table 2 Genes and gene expression assays analyzed in this study

Gene symbol	Name	Task	TaqMan Assay number	Amplicon (bp)	Slope	R²	Efficiency
ENO2	Enolase 2 (gamma, neuronal)	Reference gene	Hs00157360_m1	77	−3.356	0.994	98.61
GAPDH	Glyceraldehyde-3-phosphate dehydrogenase	Reference gene	4333764 F	122	−3.313	0.996	100.36
GFAP	Glial fibrillary acidic protein	Upregulation marker	Hs00909236_m1	59	−3.414	0.999	96.29
HPRT1	Hypoxanthine phosphoribosyltransferase 1	Reference gene	4333768 F	100	−3.306	0.988	100.67
KL	Klotho	Target	Hs00183100_m1	74	−3.396	0.998	97.02
NFKB1	Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1	Target	Hs00765730_m1	66	−3.388	0.997	97.30
TBP	TATA box binding protein	Reference gene	Hs99999910_m1	127	−3.407	0.993	96.57
TNF	Tumor necrosis factor	Target	Hs99999043_m1	85	−3.266	0.991	102.39

ISSN: 1742-2094