Figure 7

Analysis of major histocompatibility complex (MHC) II expression on central nervous system (CNS) antigen-presenting cells (APC) subsets. CNS mononuclear cells from neonatal (gray) and adult (black) mice immunized with complete Freund adjuvant (CFA)/myelin oligodendrocyte glycoprotein (MOG)_p35-55 10 days previously were isolated and prepared for flow cytometry. The median fluorescence intensity (MFI) of MHC II (A) and the percentage of each subset expressing MHC II (B) is shown. (C) Representative staining for MHC II on unimmunized (gray line) and immunized (black line) Gr-1^-CD11b⁺ monocytes and CD19⁺ B cells is shown. An isotype-matched control mAb was used to identify positive staining events and set the marker. Figures are representative of data obtained from at least three independent experiments.