Figure 2

S100A9 release process was dependent on transcription and translation processes in human monocytic THP-1 cells. THP-1 cells were incubated with Aβ1-42 (10 μM), actinomycin (ActD, 100 nM) or cycloheximide (CHX, 1 μM) for 24 hours. (A) Western blot analyses were conducted to determine the effects of actinomycin or cycloheximide on S100A9 release in the conditioned media, as described in Figure 1. S100A9 secretion was reduced when de novo mRNA expression and protein synthesis were inhibited. Representative gels from three experiments are shown. (B) Quantitative analysis of (A), showing the levels of S100A9 release. All data are presented as the means ± SEM (n = 3). **P <0.01, versus vehicle treated samples. ActD, actinomycin; CHX, cycloheximide.