Figure 3

3-D quantitative analyses of cerebral IgG and apoB extravasation. The optical staining intensities of IgG (A) and apoB (B) were measured with 3-D image analysis software and shown in the cortex (CTX) and hippocampal formation (HPF) of mice maintained on low-fat control chow (LF), diet enriched in saturated fat (SFA) or each diet supplemented with nutraceutical garlic extract-aged (+GEA), alpha-lipoic acid (+ALA), niacin (+Niacin), or nicotinamide (+NA) for nine months. One-way ANOVA followed by Tukey’s post hoc test was used to analyze the statistical significance at P <0.05. Data were analyzed in cortex and hippocampal formation separately, however shown as combined since the statistical significance was identical. (a) Significant difference between LF and SFA; (c) Significant difference between SFA and each nutraceutical treatment.