Figure 1

Progression of iris disease and intraocular pressure (IOP) profiles in DBA/2J and D2. C5 ^B6 mice. (A) The congenic interval surrounding C5 in D2.C5 ^B6 mice determined using markers polymorphic between D2 and B6 (see Methods). The proximal and distal breakpoints are indicated by the dotted line. The confirmed congenic interval from B6 is shown as a solid line. The size of the congenic interval is between 12 Mb and 19 Mb, which equates to less than 0.5% of the mouse genome. Analysis of known variations between D2 and B6 showed that C5 is the only gene in this region that has a null mutation in D2 compared with B6. (B) Eye examinations with broad-beam illumination (top) and transillumination (bottom) found no obvious differences in iris disease progression between D2.C5 ^B6 and DBA/2J mice. (C) Age-dependent elevation of IOP was found in both D2 and D2.C5 ^B6 mice (red) compared with young D2 mice (black). Box plots were generated using JMP version 7.0. The ends of each box represent the 75th and 25th percentile. The lines across the middle of each box indicate the median value. The whiskers extending from either end indicate the extent of the data points. Values falling outside the whiskers are considered outliers. The green diamonds indicate the mean and the 95% confidence interval.