Table 1 Primers used for RT-PCR
From: Dual effects of noradrenaline on astroglial production of chemokines and pro-inflammatory mediators
Gene name | Forward primer | Reverse primer |
|---|---|---|
CX3CL1 | 5′-AATCCCAGTGACCTTGCTCATCCA | 5′-TGGACCCATTTCTCCTTTGGGTCA |
CCL2 | 5′-TGCTGTCTCAGCCAGATGCAGTTA | 5′-TACAGCTTCTTTGGGACACCTGCT |
CCL6 | 5′-TGTTCCAGCAGGGCATCTTCTTCT | 5′-GCCTCATTTGCATGGAGAGCCATT |
CCL7 | 5′-GGACCAATTCATCCACTTGCTGCT | 5′-TCTGATGGGCTTCAGCACAGACTT |
CCL12 | 5′-TGAGTCCTCCAGCTCTCATTCCAA | 5′-TGAACACTGAATCTGGTCCAGCCA |
CXCL16 | 5′-TGTCGCTGGAAGTTGCTACTGTGA | 5′-TCTTGGACTGCAACTGGAACCTGA |
IL-1β | 5′-ACCTGCTAGTGTGTGATGTTCCCA | 5′-AGGTGGAGAGCTTTCAGCTCACAT |
TNFα | 5′-CTGGCCAATGGCATGGATCTCAAA | 5′-AGCCTTGTCCCTTGAAGAGAACCT |
IFNγ | 5′-AAAGACAACCAGGCCATCAGCAAC | 5′-TCTGTGGGTTGTTCACCTCGAACT |
COX-2 | 5′-GCATTCTTTGCCCAGCACTTCACT | 5′-TTTAAGTCCACTCCATGGCCCAGT |
GAPDH | 5′-TGCACCACCAACTGCTTAGC | 5′-GGCATGGACTGTGGTCATGAG |