Figure 3

The nigrostriatal pathway and microglial substantia nigra (SN) content are unaltered in DAP12-deficient mice. (A) Representative photomicrographs of striatal sections immunostained for tyrosine hydroxylase (TH) from wild-type (WT) and DAP12^KI mice. Right panel: quantification of striatal TH immunoreactivity (optical density). Bars represent the mean optical density. Scale bar: 100 μm. (B) Representative photomicrographs of mesencephalic sections immunostained for TH from WT and DAP12^KI mice. Right panel: quantification of TH⁺ dopaminergic neurons in the SN pars compacta. Bars represent the mean number of total nigral TH⁺ neurons. Scale bar: 300 μm. (C) Representative photomicrographs of mesencephalic sections from WT and DAP12^KI mice and immunostained for the microglial marker Iba1. Dashed boxes delineate the area for higher magnification views shown on the right. Scale bar: left, 400 μm; right, 50 μm. Right panel: quantification of Iba1⁺ microglial cells in the SN pars compacta of WT and DAP12^KI mice. Bars represent the mean number of Iba1⁺ microglial cells in the SN pars compacta.