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Table 1 Antibodies used for immunocytochemistry

From: M-CSF increases proliferation and phagocytosis while modulating receptor and transcription factor expression in adult human microglia

Antibody

Company

Catalogue #

Dilution

Rabbit anti-PU.1

Cell Signaling

2258

1:500

Mouse anti-CD45

Abcam

ab8216

1:500

Rabbit anti-CSF-1R

Santa Cruz

Sc-692

1:50

Mouse anti-HLA-DP, DQ, DR

Dako

M0775

1:500

Mouse anti-GFAP

Sigma-Aldrich

G3893

1:5000

Mouse anti-Prolyl 4-hydroxylase

Dako

M0877

1:1000

Rabbit anti-DAP12

Santa Cruz

Sc-20783

1:500

Mouse anti-C/EBPβ

Santa Cruz

Sc-7962

1:250

Mouse anti-IGF-1R

Millipore

MAB1120

1:50

Mouse anti-BrdU

Roche

11170376001

1:500

Rabbit anti-Ki67

Dako

A0047

1:500

Goat anti-rabbit IgG Alexa Fluor® 594

Invitrogen

A11012

1:500

Goat anti-mouse IgG Alexa Fluor® 488

Invitrogen

A11001

1:500

Goat anti-mouse IgG Alexa Fluor® 594

Invitrogen

A11005

1:500

Goat anti-rabbit IgG Alexa Fluor® 488

Invitrogen

A11008

1:500

Goat anti-rabbit biotinylated

Sigma-Aldrich

B7389

1:500

Goat anti-mouse biotinylated

Sigma-Aldrich

B7264

1:500

ExtrAvidin-HRP

Sigma-Aldrich

E2886

1:500