Figure 3

TNF-α and IL-1β mRNA and protein expression in the peri-ischemic brain tissue. Panels A and B show the graphical representation of the fold changes in TNF-α and IL-1β mRNA, respectively. They show that 10% HS significantly decreased TNF-α and IL-1β mRNA expression at 12 h after MCAO. Panels C and D show TNF-α (30 kDa), IL-1β (17 kDa) and GAPDH (37 kDa) immunoreactive bands, respectively. Panels E and F show the optical density of TNF-α and IL-1β expression in an ischemic 12-h group is significantly up-regulated when compared with the sham-operated group (#P <0.05, n = 6 per group); however, the optical density of TNF-α and IL-1β in the ischemic 12-h + 10% HS group is significantly decreased after 10% HS treatment (*P <0.05, n = 6 per group). IL-1β, interleukin-1 beta; HS, hypertonic saline; MCAO, middle cerebral artery occlusion; TNF-α, tumor necrosis factor-alpha.