Figure 11
From: Glimepiride reduces CD14 expression and cytokine secretion from macrophages
CD14 mediates cytokine secretion from peptide stimulated RAW 264 cells. (A) The concentrations of TNF produced by RAW 264 cells pre-treated with antiserum to CD14 (■), PrPC (□) or CD55 (striped bars) and incubated with 50 μM PrP82-146, Aβ1–42 or αSN. Values are means ± SD from triplicate experiments performed 3 times, n = 9. *TNF significantly less than those of control cells incubated with peptides. (B) The concentrations of TNF produced by microglial cells derived from CD14 wild type (□) or CD14 knockout (■) mice incubated with PrP82-146 or Aβ1–42. Values are means ± SD from triplicate experiments performed 3 times, n = 9. *TNF significantly less than those from CD14 wild type cells incubated with peptides.