Figure 4
Effect of ent -Sauchinone on expression of BACE1, C99, and Aβ 1-42 , β-secretase activity and Aβ 1-42 level. Expressions of BACE1 and C99 were detected by western blotting using specific antibodies in astrocytes (A) and microglial BV-2 cells (B). Each blot is representative of three experiments. β-Actin protein was used here as an internal control. Co-treatments with ent-Sauchinone and LPS for 24 hours were used. Media were collected to determine Aβ1-42 secretion by ELISA from astrocytes (C) and microglial BV-2 cells (D). Values represent the mean ± S.E. of three independent experiments with triplicate. The activities of β-secretasewere assessed using commercially available assay kits as described in Materials and Methods (E and F). Values represent mean ± S.E. for three independent experiments performed in triplicate. *indicates significantly different from the control group (P <0.05.. #indicates significantly different from the LPS-treated group (P <0.05.. Values above of each figure mean quantified relative expression of the proteins.