Figure 3

Mepazine inhibits MALT1 substrate cleavage and reduces NF-κB and JNK activation in T cells. (A-B) Jurkat T cells and (C) purified primary CD4+ T cells were stimulated with PMA plus ionomycin for indicated time points. Where indicated, mepazine was added 90 minutes prior to stimulation. Cleavage of MALT1 substrates CYLD, A20 and BCL10 was analyzed via western blot (A), and lysates were analyzed for IκBα, phospho-IκBα, JNK, phospho-JNK, p38, phospho-p38, ERK, phospho-ERK and actin (B-C).