Figure 4

Cytokine expression in transient global cerebral ischemia and CX3CR1 deficiency was detected using ELISA. (A) Significant increase in IL-1β expression was seen following transient global cerebral ischemia, which was amplified by CX3CR1 deficiency. Silencing of CX3CR1 function in sham rats also resulted in increased IL-1β expression. (B) Expression of TNF-α also increased significantly following ischemic injury and intensified by CX3CR1 deficiency. Low level TNF-α expression was seen in the sham animals and no significant group difference was seen. (C) Ischemic injury also significantly elevated the expression of the anti-inflammatory cytokine IL-4 but the upregulation was mitigated by CX3CR1 deficiency. Low level IL-4 expression was seen in the sham animals and no significant group difference was seen. (D) Similarly, IL-10 expression significantly increased after ischemic injury but the upregulation was mitigated by CX3CR1 deficiency. Low level IL-10 expression was seen in the sham animals and no significant group difference was seen. *P <0.05 and **P <0.01.