Figure 3

Diminished T-cell responsiveness in irf3 ^−/− mice with experimental autoimmune encephalomyelitis. Spleen cells from interferon regulatory factor 3–knockout (irf3 ^−/−) and wild-type (WT) mice (n = 5 or 6 mice/group) harvested 18 to 22 days after immunisation were cultured in triplicate in the presence of MOG_35–55 or anti-CD3/anti-CD28. (A) After 48 hours of culture, proliferative responses to myelin oligodendrocyte glycoprotein (MOG_35–55) or anti-CD3/anti-CD28 were determined. Proliferative responses are shown as mean counts per minute (cpm) ± SEM. (B) After 72 hours of culture, interleukin-17 (IL-17) production in response to MOG_35–55 or anti-CD3/anti-CD28 was determined in supernatants by enzyme-linked immunosorbent assay. One representative experiment of two is shown.