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Table 1 BV2 microglia in three-dimensional (3D) cultures show increased expression of IL-6, TNF-α and MCP-1 at 48 hours post-lipopolysaccharide (LPS) stimulation

From: A three-dimensional collagen construct to model lipopolysaccharide-induced activation of BV2 microglia

 

Untreated

LPS-treated

 

IL-6

TNF-α

MCP-1

IL-6

TNF-α

MCP-1

Monolayer

1.7 ± 0.3

252.3 ± 120.5

9 447.0 ± 226.8

2 476.0 ± 833.6**

3 823.0 ± 1189.0**

10 459.0 ± 1, 639.0ns

Coated monolayer

1.6 ± 0.99

217.6 ± 181.8

9 231.0 ± 2268.0

1 764.0 ± 324.3**

2 357.0 ± 500.9**

10 936.0 ± 1292.0ns

3D

0.7 ± 0.8

8.1 ± 3.1

284.0 ± 73.5

1 999.0 ± 685.2*

1 744.0 ± 911.6*

5 403.0 ± 517.6*

  1. Values are expressed in pg/ml, and assayed using the Cytometric Bead Array. Data are mean ± SD from five independent experiments. *p < .05, **p < .01; two-tailed Mann-Whitney U test, as compared to untreated controls. ns, not significant.
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Journal of Neuroinflammation

ISSN: 1742-2094