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Table 1 BV2 microglia in three-dimensional (3D) cultures show increased expression of IL-6, TNF-α and MCP-1 at 48 hours post-lipopolysaccharide (LPS) stimulation

From: A three-dimensional collagen construct to model lipopolysaccharide-induced activation of BV2 microglia

  Untreated LPS-treated
  IL-6 TNF-α MCP-1 IL-6 TNF-α MCP-1
Monolayer 1.7 ± 0.3 252.3 ± 120.5 9 447.0 ± 226.8 2 476.0 ± 833.6** 3 823.0 ± 1189.0** 10 459.0 ± 1, 639.0ns
Coated monolayer 1.6 ± 0.99 217.6 ± 181.8 9 231.0 ± 2268.0 1 764.0 ± 324.3** 2 357.0 ± 500.9** 10 936.0 ± 1292.0ns
3D 0.7 ± 0.8 8.1 ± 3.1 284.0 ± 73.5 1 999.0 ± 685.2* 1 744.0 ± 911.6* 5 403.0 ± 517.6*
  1. Values are expressed in pg/ml, and assayed using the Cytometric Bead Array. Data are mean ± SD from five independent experiments. *p < .05, **p < .01; two-tailed Mann-Whitney U test, as compared to untreated controls. ns, not significant.