Skip to main content
Figure 2 | Journal of Neuroinflammation

Figure 2

From: NitroDIGE analysis reveals inhibition of protein S-nitrosylation by epigallocatechin gallates in lipopolysaccharide-stimulated microglial cells

Figure 2

Identification and quantification of SNOC-induced protein S - nitrosylation in BV-2 cells by NitroDIGE. (A) Workflow of NitroDIGE to identify and quantify SNO-proteins. Pooled internal standard and individual samples are labeled with Cy3 and Cy5, respectively, and subjected to 2-DE. 2-DE gel fluorescence is detected using a Fuji 5000 scanner and quantified by the SameSpots software. On a corresponding zinc-staining gel, selected fluorescence intensity-differential spots are excised for MS analysis. (B) NitroDIGE analysis of protein S-nitrosylation in ex vivo SNOC-treated BV-2 cells. Following the work flow above, untreated control and SNOC-treated samples in biological triplicate were labeled with CyDye™ and resolved on six different gels. A representative gel from each group is shown. (C) Quantitative analysis with the SameSpots software revealed 47 spots with significant fluorescence intensity changes between SNOC-treated and untreated control samples (fold change >1.3, P <0.05). (D) Quantification results for spot #43 are displayed as an example.

Back to article page

Journal of Neuroinflammation

ISSN: 1742-2094