Figure 4

Repetitive hypoxic preconditioning induces an immunosuppressive phenotype prior to stroke. B cells isolated from spleen were analyzed with microarray, showing (A) distinct clustering of repetitive hypoxic preconditioning (RHP)-treated mice compared to controls (21 %; n = 5/group). (B) Top 10 up- and downregulated genes, and (C) top activated canonical pathways are shown. Gene products are shown in bar graph form as percentages of all genes associated with a listed pathway (upper axis). Gene numbers up/downregulated are located in the bars, with the total number of genes within the pathway shown to the right. Expression changes are shown as either upregulated (red bars) or downregulated (green bars), and pathways with B-T cell interactions are marked by a cross. Respective P values are indicated by orange squares (lower axis). Replicate samples on the microarray chip are indicted with (R) in panel A.