Figure 4

Decreased Cdk5 and p35 protein expression and Cdk5 activity in the brain of Cdk5 cKO2 and dKO mice. (A) Representative Western blot analysis showing Cdk5 protein levels in brain cortex of control, Cdk5 cKO2 and dKO mice at P17. Lower panel showed a quantification of Western blots for Cdk5. α-tubulin was used as loading control. (B) Representative Western blot analysis showing p35 protein levels in brain cortex of control, Cdk5 cKO2 and dKO mice at P17. Lower panel showed a quantification of Western blots of p35. α-tubulin was used as loading control. (C) Cdk5 kinase activity was measured from immunoprecipitates of proteins obtained from brain cortex of control, Cdk5 cKO2 and dKO mice at P17. All data are presented as the mean and SEM (n = 3 to 12). *P < 0.05, **P < 0.01, ***P < 0.001 (t-test).