Figure 3

STAT6-deficient C57Bl/6 mice are fully protected by atorvastatin treatment. Signal transducer and activator of transcription 6 (STAT6)-deficient and wild-type (WT) C57Bl/6 mice were fed daily with atorvastatin (AT; 1 mg/kg/day or 10 mg/kg/day) or vehicle (phosphate-buffered saline (PBS)) starting either 2 days prior to immunization (prevention, left panels) or after experimental autoimmune encephalomyelitis (EAE) was fully established (reversal, right panels). (a) Mice were evaluated daily for clinical EAE symptoms. (b) At study termination, mice were sacrificed and evaluated for histologic signs of EAE. Shown is the number of inflammatory lesions per three representative (cervical, thoracal, lumbal) hematoxylin and eosin-stained spinal cord sections. Twelve mice/group were used; two independent studies were performed for each setting.