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Table 2 Primers used for RT-PCR a

From: Chronic stress enhances microglia activation and exacerbates death of nigral dopaminergic neurons under conditions of inflammation

Target mRNA Forward (F) and reverse (R) primers Reference
TNF-α F: 5′-TACTGAACTTCGGGGTGATTGGTCC-3′ [53]
  R: 5′-CAGCCTTGTCCCTTGAAGAGAACC-3′  
IL-6 F: 5′-AAAATCTGCTCTGGTCTTCTGG-3′ [54]
  R: 5′-GGTTTGCCGAGTAGACCTCA-3′  
β-actin F: 5′-TGTGATGGTGGGAATGGGTCAG-3′ [55]
  R: 5′-TTTGATGTCACGCACGATTTCC-3′  
IL-1β F: 5′-CAGGATGAGGACATGAGCACC-3′ [55]
  R: 5′-CTCTGCAGACTCAAACTCCAC-3′  
iNOS F: 5′-CCTCCTCCACCCTACCAAGT-3′ [56]
  R: 5′-CACCCAAAGTGCTTCAGTCA-3′  
CD200 F: 5′-TGTTCCGCTGATTGTTGGC-3′ [57]
  R: 5′- ATGGACACATTACGGTTGCC-3′  
CX3CR1 F: 5′-GGC CTT GTC TGA TCT GCT GTT TG-3′ [58]
  R: 5′- AAT GCT GAT GAC GGT GAT GAA GAA-3′  
MCP-1 F: 5′-AGCATCCACGTGCTGTCTC-3′ Universal ProbeLibraryb
  R: 5′-GATCATCTTGCCAGTGAATGAG-3′  
  1. a CX3CR1, chemokine (C-X3-C motif) receptor 1; IL-6, Interleukin 6; iNOS, inducible nitric oxide synthase; MCP-1, monocyte chemoattractant protein 1; TNF, Tumour necrosis factor. bRoche Applied Science (Indianapolis, IN, USA).