Figure 5

Apoptotic bodies are taken up by MECs into the lysosomal compartment. Ben-Men-I cells were pulsed with CFSE-labeled apoptotic U-937 cells for 5 min before fixation at the indicated time point. Subcellular localization of apoptotic bodies inside MECs was analyzed using confocal microscopy at the indicated time points by staining against the early endosomal marker EEA-1 (A), the late endosomal/lysosomal marker LAMP-1 (B), or by using LysoTracker (C) as marker for acidified lysosomal compartments. Please note the co-localization of apoptotic bodies (labeled green) with the early endosomal marker EEA-1 (red) at early time points as well as the overlap between apoptotic cells and lysosomal markers (LAMP-1 and LysoTracker - red) in later time points.