Table 2 Antibodies used for Western blotting
Antigen | Type | Dilution | Source |
|---|---|---|---|
Astrocyte | Â | Â | Â |
 Cx43 | Rabbit polyclonal | 1:1000 | Abcam, Cambridge, UK |
 Cx30 | Rabbit polyclonal | 1:200 | Sigma Aldrich, St Louis, USA |
 GFAP | Rabbit polyclonal | 1:1000 | DakoCytomation, Glostrup, Denmark |
 EAAT2 | Mouse monoclonal | 1:50 | Novocastra, Newcastle upon Tyne, UK |
Oligodendrocyte/myelin | Â | Â | Â |
 Cx32 | Rabbit polyclonal | 1:250 | Life Technologies, California, USA |
 Cx47 | Mouse monoclonal | 1:200 | Life Technologies, California, USA |
 MOG | Rabbit polyclonal | 1:1000 | Sigma Aldrich, St Louis, USA |
Housekeeping protein | Â | Â | Â |
 GAPDH | Mouse monoclonal | 1:10000 | Santa Cruz Biotechnology, California, USA |