Figure 2

Oxygen glucose deprivation (OGD) stimulates a classical innate inflammatory cascade involving an up regulation in IL-1β, IL-6 and TNF-α. M17 cells were subjected to 4.5 h of OGD and reperfused for a 0 to 24 h period. Q-PCR was then performed detecting: (A) IL-1β, (B) IL-6 and (C) TNFα mRNA levels (*P <0.05, **P <0.01, ***P <0.001, n = 3, unmatched one-way ANOVA, Dunnett’s post-hoc test). Data are expressed as fold change in mRNA transcript levels in reference to no OGD control samples. Reperfusion times are indicated in hours on the x axis and graphical data are represented as mean ± SEM.