Ambroxol treatment blocks the changes in the biophysical properties of Na
1.8 in large-sized DRG neurons extracted from rats 14 days post-CFA. (A) I-V curves of Nav1.8 currents obtained from sham and inflamed large sensory neurons. (B) Histogram showing the effects of different concentrations of ambroxol (20 and 100 μM) on the increased Nav1.8 peak current induced by CFA (***P <0.001, inflamed vs. sham large sensory neurons; ###
P <0.001 compared to inflamed DRG neurons; ANOVA followed by Sidak’s MCT; n = 6–13). (C) Ambroxol also significantly inhibits the leftward shift of the activation curves of Nav1.8 current. Half-activation potential and slope factor are summarized in Additional file 3: Table S3. Note that the data corresponding to sham and day 14 after CFA treatment are the same as the ones in Figure 4.