Table 3 Antibodies for Western blot
Antibody | Host | Vendor | Dilution | Molecular weight (kDa) |
|---|---|---|---|---|
iNOS/NOS2 | Rabbit | BD Biosciences | 1:4000 | 130 |
α-Actinin | Mouse | BD Biosciences | 1:5000 | 105 |
p- NFκB p65 (Ser536) | Rabbit | Cell Signaling | 1:1000 | 65 |
NFκB p65 | Rabbit | Cell Signaling | 1:1000 | 65 |
p-PKR (Thr451) | Rabbit | Sigma-Aldrich | 1:500 | 65 |
GAPDH | Mouse | Millipore | 1:2000 | 36 |
p-eIF2α (Ser51) | Rabbit | Abcam | 1:500 | 36 |
α-rabbit-HRP conjugated | Goat | Jackson ImmunoResearch | 1:5000 | |
α-mouse-HRP conjugated | Goat | Jackson ImmunoResearch | 1:2000 |