Figure 7
Aspirin-induced inhibition of Notch activation and proinflammatory cytokine production. Rats received various aspirin treatments around a 90-minute middle cerebral arterial occlusion (MCAO). Intracerebroventricular injection of N-[N-(3,5-Difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester (DAPT) was performed immediately after the MCAO. The right frontal cortex area 1 was harvested at 3 days after the MCAO. The cytosol was prepared for Western blotting for Notch intracellular domain (NICD) and ELISA for IL-6 and IL-1β. (A) NICD expression. Top panel shows representative Western blots and bottom panel shows the quantification results. (B) IL-6 results. (C) IL-1β results. Results are the means ± SEM (n = 8). *P < 0.05, compared with sham; ^P < 0.05, compared with the animals subjected to MCAO only. ASA, acetylsalicylic acid.