Figure 2

CD11c ⁺ microglia resemble CD45 ^high CD11c ⁺ cells in expression of molecules needed for antigen presentation and T-cell activation. Isolated cells from B6 mice with severe experimental autoimmune encephalomyelitis were stained for major histocompatibility complex (MHC) class I, MHC class II, CD80 and CD86 and analyzed by flow cytometry. For comparison of central nervous system antigen presenting cells, cells were gated as CD45^dim CD11b⁺, CD11c⁻, CD45^dim CD11b⁺, CD11c⁺ and CD45^high CD11c⁺. CD11c⁺ cells from the spleen (spl. DC) were used as positive control. Data are presented as means ± SEM of three individual experiments (n = 10). ns, Non-significant differences (P > 0.05). NetMFI, Median Fluorescent Intensity – background.