Effects of deoxyglucose on neuronal density. (A) Neuronal–glial cultures were treated ± l-leucine-methyl ester (LME) for 4 hours to remove microglia, and then treated ±10 mM deoxyglucose (DOG) for 96 hours, and total (live and dead) neuronal density was counted. Prior depletion of microglia with LME prevented the neuroprotective effect of DOG. (B) Neuronal–glial cultures were untreated or treated with 10 mM DOG at 7, 8, 9 or 10 days in vitro (DIV), and then total (live and dead) neuronal density measured at 11 DIV. Data presented as mean ± standard error of the mean for ≥ 3 independent experiments. Statistically significant differences between total neuronal densities: *P < 0.05.