Figure 4
Double-labeled immunofluorescence TLR 4 receptor and specific cell population in blood spinal cord barrier after spinal cord I/R injury. (A) Representative immunohistochemical localization of capillary endothelial cells (CD31; green), pericytes (CD13; green), microglia (Iba-1; green), astrocytes (GFAP; green), and Toll-like receptor (TLR4; red) in spinal cords of sham-operated or operated rats at 12 and 48 h after the surgical procedure, respectively. Scale bars = 100 μm for the 200× images. (B) Effects of TLR4 signaling on TLR4 co-localization cells. Representative double-labeling micrographs show that intrathecal pretreatment with TAK-242, MIP, and Resveratrol before I/R injury significantly prevented microglia and astrocytes upregulated expression of TLR4 at 48 h after the surgery. Scale bars = 50 μm for the 400× images. (C) Quantification of TLR4-positive microglia and TLR4-positive astrocytes in the spinal cords. Data are presented as mean ± SEM (n = 6). **P < 0.01 vs. Sham group; ## P < 0.01 vs. I/R group; &&P < 0.05 vs. TAK group.