In vitro characterization of NSC differentiation. NSCs isolated from rats differentiate into neuron-like cells and glial cells, and express (A) NeuN (a neuron marker), (B) GFAP (an astrocyte marker), and (C) APC (an oligodendrocyte marker) as shown by immunocytochemical staining. (D) Quantitative differentiation (percent of NSCs). Scale bar = 100 μm, shown in (C). NSC, neural stem cell.