Increased mRNA expression of inflammatory markers in neonatal brain tissue following LPS stimulation. Real-time PCR analysis was used to analyze the expression of neuroinflammatory markers in brain tissue from neonatal and weanling mice at 6 hpi. Increased mRNA expression of Nos2 (iNos) (A) and Icam1 (B) mRNAs were observed in brain tissue from LPS-inoculated neonatal mice, while Csf2 (GMCSF) mRNA (C) was elevated in brain tissue from LPS-inoculated weanling mice. n = 4 mice per group. Data are presented as mean ± SEM. Statistical analysis was completed by two-way analysis of variance with Tukey’s multiple comparisons test. Significant age-specific differences in the LPS response are indicated in the figure as: ***P < 0.001.