STAT6 is required for PPARγ activation. BV-2 cells were treated with leflunomide (10 μM) or GW9662 (1 μM) 30 minutes prior to administration of RvD1 (100 nM), followed by incubation with IL-4 (10 ng/ml) for 120 minutes. PPARγ protein level in nucleus (A) and DNA binding activity (B) were analyzed using Western blot or electrophoretic mobility shift assay (EMSA). A representative result from three independent experiments is shown. Quantification of PPARγ was normalized by lamin B1. Data are presented as mean ± SEM for three independent experiments. Asterisks indicate statistically significant difference (*P < 0.05, **P < 0.01).