Cytokines levels in the brains of WT and db / db mice after WNV infection. Brains were harvested from WT and db/db mice at indicated time points and homogenized as described in the Materials and methods section. Levels of cytokines as noted in the figure (A) IL-1β, (B) TNF, (C) IL-6, (D) IFNγ, (E) IL-1α, and (F) IL-13 were measured using multiplex immunoassay and are expressed as the mean concentration (pg/g of tissues) ± SEM, representing two independent experiments (n = 7 per group). *P <0.05. SEM, standard error of mean.