Figure 3

Systemic lipopolysaccharide (LPS) recruited inflammatory cells in retina. A. Immunohistochemical staining of retinal flat mounts from P7 rats showed a massive infiltration of CD11c⁺ cells around the area of focal abnormal vascular proliferation (arrows) in central and peripheral retina. Scale bars = 200 μm. B. On P14 retina, there were focal proliferations of abnormal retinal vessels in the superficial vascular layer (arrows). In the deep layer corresponding to the superficial layer of abnormal vascular proliferation, there were scanty capillaries indicating concomitant vascular regression (dashed circle). C. In the areas of focal vascular proliferations, infiltration of CD11c⁺ cells and proliferation of GFAP-stained astrocytes were noted. Also, regression of pre-existing capillaries was accompanied as indicated by ghost sheathing of NG2-stained pericytes (arrow). Scale bar = 1000 μm.