Figure 3

Increased phospho-IκBα and decreased IκBα in choroidal-RPE tissues of CNV. A, Choroidal-RPE tissues were isolated from non-treated and laser-treated eyeballs at days three and five after photocoagulation and processed for immunoblotting. The primary antibodies phospho-IκBα (1:200) and IκBα (1:200) and the peroxidase-conjugated secondary antibody (1:5000) were used, followed by ECL detection. The optical density ratios for p-IκBα/GAPDH and IκBα/GAPDH were indicated in B, the control values were set as 100 and the values for treated conditions were normalized to the control values. The images were typical of those from three independent experiments with tissues for each condition of per experiment collected from three eyeballs. *P < 0.05 versus the corresponding controls. CNV, choroidal neovascularization; RPE, retinal pigment epithelia; IκBα, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha; ECL, enhanced chemiluminescence assay; GAPDH, glyceraldehydes 3-phosphate dehydrogenase.