Figure 3

Relative caspase-3 mRNA levels in incubated arteries. (A) evaluated in arteries incubated for 0 (fresh), 3, 6 or 24 hours in serum-free DMEM (0 hours was used as control) and (B) in arteries incubated for 24 hours with or without CaMKII inhibitor (KN93, 10⁻⁵ M) or MEK1/2 inhibitor (U0126, 10⁻⁵ M) added at 0 or 6 hours after initiating incubation (24-hour-incubated arteries with vehicle were used as control). Data are presented as mean ± SEM relative to (elongation factor-1) EF-1, n = 4–6. *P <0.05 **P <0.01, ***P <0.001. ^P <0.05 represents significant difference between 0 and 6 hours in arteries incubated with KN93. Dulbecco’s modified eagle’s medium (DMEM), calcium calmodulin-dependent protein kinase II (CAMKII).