Figure 4

Detection of SEAP in the culture medium of PC12 cells transfected with a SEAP reporter gene construct under the transcriptional control of enhancers specific for NF-κB, AP-1 or CRE. pTal is the non-enhanced control SEAP reporter vector. Twenty-four hr after transfection, cells were treated with vehicle (Control), 10 ng/ml NGF, 10 ng/ml TNFα or NGF plus TNFα (Both) and the presence of SEAP in the culture medium assayed 3 hr (Top) or 12 hr (Bottom) thereafter. Results are normalized to control cells in each transfection group (N = 3). * and #: p < 0.05 vs. control and TNFα-alone, respectively (two-tailed unpaired Student's t-test). Results shown are representative of 3 replicate experiments.